fibroblast culture medium fcm (Lonza)
Structured Review

Fibroblast Culture Medium Fcm, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibroblast+culture+medium+fcm/pmc10902434-153-5-13?v=Lonza
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Reconstruction of the human nipple–areolar complex: a tissue engineering approach"
Article Title: Reconstruction of the human nipple–areolar complex: a tissue engineering approach
Journal: Frontiers in Bioengineering and Biotechnology
doi: 10.3389/fbioe.2023.1295075
Figure Legend Snippet: Human fibroblast seeding on the hypodermal side of d-NACs. (A) H&E staining of adherent human fibroblasts (arrows) on the acellular hypodermal side and, in some locations, adherent to the epidermal side of d-NACs after 7 days of static culture (scale bar = 200 μm). (A’) Higher magnification of the H&E-stained section highlighting adherent fibroblasts forming several cell layers on the hypodermal side of the scaffold (scale bar = 100 μm). (B) Live/dead staining of seeded fibroblasts shows a high viability on day 7 of the culture on the scaffold (living cells = green and dead cells = red) (scale bar = 500 μm). (C) Cell viability of the seeded dermis: ECM- red and control wells- blue. The results are expressed as the mean cell viability. Error bars: SD; ns = not significant. (D) A PrestoBlue cell viability assay realized on seeded d-NACs (red, n = 3) and control culture wells (blue, n = 3) attests the biocompatibility of the produced scaffolds by the increase in metabolic activity during the 7 days of culture. The results are expressed as the mean fluorescence intensity. Error bars: SD; ** p < 0.01; ns = not significant.
Techniques Used: Staining, Control, Viability Assay, Produced, Activity Assay, Fluorescence
Figure Legend Snippet:
Techniques Used: Immunofluorescence, Immunohistochemistry, Immunopeptidomics, Saline, Standard Deviation